Anti-Cyclic Citrullinated Peptide (CCP) Antibody ELISA Kit

Setsi sena se lemoha li-anti-cyclic citrullinated peptide antibodies (CCP antibodies) ka lisampole tsa serum ea batho ho latela mokhoa o sa tobang, ka li-antigen tsa peptide tse hloekisitsoeng tsa cyclic citrullinated tse sebelisoang e le antigen ea ho roala.

Ts'ebetso ea tlhahlobo e qala ka ho kenyelletsa sampole ea serum lilibeng tsa karabelo tse kentsoeng pele ka li-antigen tse hloekisitsoeng tse boletsoeng ka holimo, 'me e lateloe ke nako ea ho incubation. Nakong ea ts'ebetso ena, haeba li-antibodies tsa CCP li le teng sampuling, li tla lemoha ka ho khetheha le ho tlama li-antigen tsa cyclic citrullinated peptide tse koahetsoeng ka li-microwells, ho etsa li-antigen-antibody complexes tse tsitsitseng. Ho etsa bonnete ba ho nepahala ha mehato e latelang, likarolo tse sa tlamisoang lilibeng tsa karabelo li tlosoa ka mokhoa oa ho hlatsoa, ​​o thusang ho felisa tšitiso e ka 'nang ea e-ba teng ho tsoa linthong tse ling tsa serum.

Ka mor'a moo, li-conjugate tsa enzyme li kenngoa lilibeng tsa karabelo. Kamora ho kenella ka lekhetlo la bobeli, likhokahano tsena tsa enzyme li tla ikamahanya ka ho khetheha le li-antigen-antibody complexes tse seng li ntse li le teng, ho theha setsi se seholo sa 'mele sa ho itšireletsa mafung se kenyelletsang antigen, antibody, le enzyme conjugate. Ha tharollo ea TMB substrate e kenngoa tsamaisong, enzyme e ka har'a conjugate e susumetsa karabelo ea lik'hemik'hale ka karoloana ea TMB, e bakang phetoho e bonahalang ea 'mala. Matla a karabelo ena ea 'mala a amana ka kotloloho le palo ea li-antibodies tsa CCP tse teng sampoleng ea pele ea serum. Qetellong, sebali sa microplate se sebelisetsoa ho lekanya ho monya (A boleng) ba motsoako oa karabelo. Ka ho sekaseka boleng bona ba ho monya, boemo ba li-antibodies tsa CCP sampoleng bo ka khethoa ka nepo, ho fana ka motheo o tšepahalang oa tlhahlobo le tlhahlobo ea bongaka e nepahetseng.