Anti-Islet Cell (ICA) Antibody ELISA Kit

Tlhaloso e Khutšoanyane:

Setsi sena se etselitsoe tlhahlobo ea in vitro ea boleng ba anti-islet cell antibody (ICA) serum ea motho. Tleliniki, e sebelisoa haholo e le sesebelisoa se thusang sa tlhahlobo ea lefu la tsoekere la Mofuta oa 1 (T1DM).

Li-antibodies tsa lisele tsa Islet ke li-autoantibodies tse shebileng li-antigen tse holim'a metsi kapa ka har'a lisele tsa pancreatic islet, haholo-holo lisele tsa β. Ho ba teng ha bona ho amana haufi-ufi le tšenyo ea autoimmune ho lisele tsa islet, e leng karolo ea bohlokoa ea lefu la T1DM. Mehatong ea pele ea T1DM, le pele matšoao a hlakileng a kliniki a kang hyperglycemia a hlaha, ICA e ka fumanoa hangata serum, e leng se etsang hore e be letšoao la bohlokoa la ho itšireletsa mafung bakeng sa lefu lena.

Bakeng sa batho ba nang le nalane ea lelapa la lefu la tsoekere kapa ba nang le matšoao a pele ho lefu la tsoekere, ho lemoha maemo a ICA ho thusa ho lekola kotsi ea ho ba le T1DM. Ho feta moo, ho bakuli ba nang le lisosa tse sa hlakang tsa hyperglycemia, lithuso tsa tlhahlobo ea ICA ho khetholla T1DM ho mefuta e meng ea lefu la tsoekere, ka hona e tataisa ho etsoa ha merero e nepahetseng ea kalafo. Ka ho beha leihlo liphetoho maemong a ICA, e ka fana ka litšupiso bakeng sa ho lekola tsoelo-pele ea tšenyo ea lisele tsa islet le katleho ea mehato ea ho kenella.

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Molao-motheo

Setsi sena se lemoha li-antibodies tsa lisele tsa islet (ICA) ka lisampole tsa serum ea batho ho latela mokhoa o sa tobang, ka li-antigen tsa lisele tse hloekisitsoeng tse sebelisoang e le antigen ea ho roala.

Ts'ebetso ea tlhahlobo e qala ka ho kenyelletsa sampole ea serum ho liliba tsa karabelo tse kentsoeng pele ka antigen, e lateloe ke ho kenya. Haeba ICA e le teng ka sampuli, e tla tlama ka ho khetheha ho li-antigen tsa lisele tsa islet tse koahetsoeng ka liliba, ho etsa li-antigen-antibody complexes tse tsitsitseng. Likarolo tse sa tlamisoang li tlosoa ka ho hlatsuoa ho netefatsa ho nepahala ha liketso tse latelang.

Ka mor'a moo, li-conjugate tsa enzyme li kenngoa lilibeng. Ka mor'a mohato oa bobeli oa ho incubation, li-conjugate tsa enzyme li tlama ho metsoako e teng ea antigen-antibody. Ha tharollo ea TMB substrate e hlahisoa, enzyme e rarahaneng e susumetsa karabelo ka TMB, e bakang phetoho e bonahalang ea 'mala. Qetellong, sebali sa microplate se sebelisetsoa ho lekanya ho monya (A value), e lumellang hore ho be le boikemisetso ba maemo a ICA ka sampuli ho latela matla a karabelo ea 'mala.

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Tlhaloso ea Sehlahisoa

Molao-motheo	Tekolo ea enzyme e amanang le immunosorbent
Mofuta	E sa tobangMokhoa
Setifikeiti	NMPA
Mohlala	Serum ea motho / plasma
Tlhaloso	48T /96T
Mocheso oa polokelo	2-8℃
Shelf bophelo	12likhoeli