Enterovirus 71(EV71) IgM ELISA Kit

Tlhaloso e Khutšoanyane:

Enterovirus 71 IgM (EV71-IgM) ELISA kit ke enzyme e amanang le immunosorbent assay bakeng sa ho lemoha boleng ba li-antibodies tsa sehlopha sa IgM ho Enterovirus 71 ka serum ea motho kapa plasma.E reretsoe ho sebelisoa lilaboratoring tsa bongaka bakeng sa tlhahlobo le taolo ea bakuli ba amanang le tšoaetso ea Enterovirus 71.

Motho ea bitsoang enterovirus 71 (EV71), setho se secha ka ho fetisisa sa Enteroviridae, ke sesosa se tloaelehileng sa lefu la letsoho, leoto le molomo (HFMD) 'me ka linako tse ling le amahanngoa le mafu a matla a tsamaiso ea methapo ea bohareng.Hangata tšoaetso e fihla sehlohlolong ka Phuptjane le Phupu.Tšoaetso ea EV71 e ka ba ntle le matšoao kapa e ka baka letšollo le makhopho.Bana ba ka tlase ho lilemo tse hlano ba kotsing e kholo ea ho ba le tšoaetso e matla ho feta batho ba baholo le bana ba baholoanyane.

EV71 e fetisoa ho tloha ho motho ho ea ho e mong ka ho kopana ka kotloloho le ka ho silafala matsohong kapa linthong tse nang le mantle a tšoaelitsoeng.Linko le 'metso, mathe kapa maro a tsoang machacheng le tsona li ka jala kokoana-hloko.

EV71 e ka aroloa 'me ea bonoa 'metsong le litulong ho sebelisoa tlhahlobo ea PCR.RNA ea kokoana-hloko e fumanoe mokelikeli oa vesicle ea letlalo, mali le moroto.Tlhahlobo ea pejana le e bonolo e ka finyelloa ho sebelisoa litlhahlobo tsa serological tse khethehileng tsa EV71, ho kenyeletsoa liteko tse khethehileng tsa IgM antibody.

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Lintlha tsa Sehlahisoa

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Molao-motheo

Kit ena e lemoha antibody ea Enterovirus 71 IgM (EV71-IgM) ka har'a serum ea motho kapa lisampole tsa plasma, li-polystyrene microwell strips li koahetsoe pele ka li-antibodies tse lebisitsoeng ho liprotheine tsa immunoglobulin M tsa motho (anti-μ chain) . ha li hlahlojoa, li-antibodies tsa IgM tsa mohlala li ka tšoaroa, 'me likarolo tse ling tse sa tlangoa (ho kenyeletsa le li-antibodies tse khethehileng tsa IgG) li tla tlosoa ka ho hlatsoa.Mohato oa bobeli, HRP (horseradish peroxidase) -antigens conjugated e tla itšoara feela ka li-antibodies tsa EV71 IgM.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (anti-μ) - (EV71-IgM) - (EV71-Ag-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e hokahaneng le e rarahaneng e etsa hore ho be le karabelo ea moqapi oa mebala. ho hlahisa ntho e putsoa, eketsa 50μL ea Stop Solution, 'me u fetohe mosehla.Boteng ba ho monya ha antibody ea EV71-IgM sampoleng ho ile ha khethoa ke sebali sa microplate.

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Tlhaloso ea Sehlahisoa

Molao-motheo	Tekolo ea enzyme e amanang le immunosorbent
Mofuta	Mokhoa oa ho nka
Setifikeiti	CE
Mohlala	Serum ea motho / plasma
Tlhaloso	48T / 96T
Mocheso oa polokelo	2-8℃
Shelf bophelo	Likhoeli tse 12