Kokoana-hloko ea Hepatitis D IgM ELISA Kit

Setsi sena se lemoha kokoana-hloko ea hepatitis D IgM antibody (HDV-IgM) ka serum ea motho kapa lisampole tsa plasma, li-polystyrene microwell strips li koahetsoe pele ka li-antibodies tse lebisitsoeng ho liprotheine tsa immunoglobulin M (anti-μ chain).Ka mor'a ho qala ho kenyelletsa li-serum kapa tsa plasma tse lokelang ho hlahlojoa, li-antibodies tsa IgM tsa mohlala li ka tšoaroa, 'me likarolo tse ling tse sa tlamisoang (ho kenyeletsa le li-antibodies tse khethehileng tsa IgG) li tla tlosoa ka ho hlatsoa.Mohato oa bobeli, HRP (horseradish peroxidase) -li-antigen tse kopantsoeng li tla itšoara feela ka li-antibodies tsa HDV IgM.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (anti-μ) - (HDV-IgM) - (HDV Ag-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e amanang le e rarahaneng e etsa hore ho be le karabelo ea moqapi oa mebala ho. hlahisa ntho e putsoa, ​​eketsa 50μl ea Stop Solution, 'me u fetohe mosehla.Boteng ba ho monya ha antibody ea HDV-IgM sampoleng ho ile ha khethoa ke sebali sa microplate.