Kokoana-hloko ea Hepatitis E IgM ELISA Kit

Tlhaloso e Khutšoanyane:

Hepatitis E kokoana-hloko IgM Test Cassette e sebelisoa bakeng sa ho lemoha ka mokhoa o nepahetseng li-antibodies tsa Hepatitis E Virus IgM ka serum ea motho, plasma (EDTA, heparin, sodium citrate) kapa mali a feletseng (EDTA, heparin, sodium citrate).Teko e lokela ho sebelisoa e le thuso ho hlahlobisiseng lefu la sebete la kokoana-hloko, le bakoang ke kokoana-hloko ea Hepatitis E.

Kokoana-hloko ea Hepatitis E (HEV) ke kokoana-hloko ea RNA e sa koalehang, e nang le khoele e le 'ngoe e fetisoang ka tsela ea mantle le molomo, tšelo ea mali le mohlomong le lesea.Ho tšoaetsoa ke HEV ho baka acute sporadic and epidemic viral Hepatitis mme ho baka mafu a sebete a hlobaetsang kapa a manyenyane a tšoanang le Hepatitis A. Leha ho na le mefuta e mene e meholo ea lefutso ea HEV, ho na le serotype e le 'ngoe feela.

Tšoaetso ea HEV bathong e hlahisa li-antibodies tsa IgM, IgA le IgG.HEV-IgM le HEV-IgA positivity ke sesupo sa tšoaetso e matla kapa ea morao tjena ea HEV.Hore na anti-HEV-IgM le anti-HEV-IgA li ntle ho e le 'ngoe kapa ka bobeli, ke sesupo sa tšoaetso ea morao-rao ea HEV.Ho ba teng ha tšoaetso ea morao tjena ea HEV, ho kopantsoe le ts'ebetso ea sebete, ho ka sebelisoa ho fumana hore na tšoaetso e matla kapa e sa tsoa feta.Ho ba teng ha tšoaetso ea HEV sebeteng ho ka sebelisoa ho fumana hore na lefu lena ke hepatitis E e matla kapa ho hlaphoheloa ho tsoa ho lefu la sebete la E.

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Molao-motheo

Setsi sena se lemoha kokoana-hloko ea hepatitis E IgM antibody (HEV-IgM) ka har'a serum ea motho kapa lisampole tsa plasma, li-polystyrene microwell strips li koahetsoe pele ka li-antibodies tse lebisitsoeng ho liprotheine tsa immunoglobulin M tsa motho (anti-μ chain).Ka mor'a ho qala ho kenyelletsa li-serum kapa tsa plasma tse lokelang ho hlahlojoa, li-antibodies tsa IgM tsa mohlala li ka tšoaroa, 'me likarolo tse ling tse sa tlamisoang (ho kenyeletsa le li-antibodies tse khethehileng tsa IgG) li tla tlosoa ka ho hlatsoa.Mohatong oa bobeli, HRP (horseradish peroxidase) -li-antigen tse kopantsoeng li tla itšoara feela ka li-antibodies tsa HEV IgM.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (anti-μ) - (HEV-IgM) - (HEV Ag-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e hokahaneng le e rarahaneng e etsa hore ho be le karabelo ea moqapi oa mebala ho. hlahisa ntho e putsoa, eketsa 50μl ea Stop Solution, 'me u fetohe mosehla.Boteng ba ho monya ha lesole la 'mele la HEV-IgM sampoleng bo ile ba khethoa ke sebali sa microplate.

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Tlhaloso ea Sehlahisoa

Molao-motheo	Tekolo ea enzyme e amanang le immunosorbent
Mofuta	Mokhoa oa ho nka
Setifikeiti	CE
Mohlala	Serum ea motho / plasma
Tlhaloso	48T / 96T
Mocheso oa polokelo	2-8℃
Shelf bophelo	Likhoeli tse 12