Measles Virus (MV) IgG ELISA Kit

Setsi sena se lemoha 'Maasles virus IgG antibody (MV-IgG) ka har'a serum ea batho kapa lisampole tsa plasma, li-polystyrene microwell strips li tlatsitsoe ka antigen ea Measles virus.Ka mor'a ho qala ho kenyelletsa li-serum kapa li-plasma tsa ho hlahlojoa tse lokelang ho hlahlojoa, li-antibodies tse tšoanang (MV-IgG-Ab & some IgM-Ab) tse teng mefuteng ea mokuli li tlamella li-antigen sebakeng se tiileng, 'me likarolo tse ling tse sa tlangoa li tla tlosoa ka ho hlatsoa.Mohato oa bobeli, HRP(horseradish peroxidase) -conjugated anti-human IgG e tla itšoara feela ka li-antibodies tsa MV IgG.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (MV Ag) - (MV-IgG) - (anti-human IgG-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e amanang le e rarahaneng e susumetsa karabelo ea moqapi oa mebala. ho hlahisa ntho e putsoa, ​​ho eketsa 50μl ea Stop Solution, le ho fetoha mosehla.