Mycoplasma Pneumonia IgM ELISA Kit

Setsi sena se lemoha Mycoplasma Pneumoniae IgM (MP-IgM) ka har'a serum ea motho kapa lisampole tsa plasma, li-polystyrene microwell strips li koahetsoe esale pele ka li-antibodies tse lebisitsoeng ho liprotheine tsa immunoglobulin M tsa motho (anti-μ chain) . , li-antibodies tsa IgM tsa mohlala li ka nkoa, 'me likarolo tse ling tse sa tlangoa (ho akarelletsa le li-antibodies tse khethehileng tsa IgG) li tla tlosoa ka ho hlatsoa.Mohato oa bobeli, li-antigen tse kopantsoeng tsa HRP (horseradish peroxidase) li tla itšoara feela ka li-antibodies tsa MP IgM.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (anti-μ) - (MP-IgM) - (MP-Ag-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e hokahaneng le e rarahaneng e susumetsa karabelo ea moqapi oa mebala. ho hlahisa ntho e putsoa, ​​eketsa 50μl ea Stop Solution, 'me u fetohe mosehla.Boteng ba ho monya ha anti-MP-IgM sampole ho ile ha khethoa ke sebali sa microplate.