Tlhahlobo ea HFRS - Hemorrhagic Fever e nang le Renal Syndrome

Ka morao

Kokoana-hloko ea Hantaan (HV) ke kokoana-hloko e ka sehloohong e ikarabellang bakeng sa Hemorrhagic Fever e nang le Renal Syndrome (HFRS). HFRS ke lefu le tšoaetsanoang lefatšeng ka bophara la zoonotic le tšoaetsanoang ka feberu, tšollo ea mali le ho fokola ha renal. Boloetse bona bo na le ts'oaetso e matla, tsoelo-pele e potlakileng, le sekhahla se phahameng sa lefu, se behang tšokelo e kholo bophelong ba sechaba. Likhoto (tse kang Apodemus agrarius le Rattus norvegicus) ke libaka tse ka sehloohong tsa polokelo le likokoana-hloko tsa HV. Phetiso ho batho e hlaha haholo ka mantle a aerosolized (moroto, mantle, mathe), ho kopana ka kotloloho, kapa ho longoa ke vector. HFRS e ka etsahala selemo ho pota, 'me batho ka kakaretso ba kotsing. Ho ea ka lipalo-palo tsa WHO, linaha tse 32 lefatšeng ka bophara li tlalehile ho qhoma ha HV, ka sekhahla se phahameng haholo Asia Bochabela, Europe le Linaheng tsa Balkan.

Li-Antibody Markers Post HV Infection

Ka mor'a tšoaetso ea HV, sesole sa 'mele oa motho se hlahisa li-antibodies tse khethehileng, haholo-holo HV-IgM le HV-IgG.

● Lithibela-mafu tsa HV-IgM: E sebetsa e le letšoao la serological la tšoaetso e qalang, hangata e hlahang matsatsing a seng makae ka mor'a matšoao a eona, 'me ke ea bohlokoa bakeng sa tlhahlobo e potlakileng.

● Lithibela-mafu tsa HV-IgG: Li hlaha hamorao ’me li ka ’na tsa tšoarella bophelo bohle, ho bontša tšoaetso ea nakong e fetileng kapa ho hlaphoheloa. Keketseho e imenneng hane kapa ho feta ea "antibody titer" ea HV-IgG lipakeng tsa lisampole tsa serum ea acute le convalescent le eona ke tlhahlobo ea ts'oaetso e matla.

Mekhoa e Tloaelehileng ea Tlhahlobo ea HV

Mekhoa ea hajoale ea laboratori ea ho lemoha HV e kenyelletsa ho aroloa ha vaerase, PCR, serological ELISA, le li-immunoassay tsa khauta tsa colloidal.

● Tloaelo ea kokoana-hloko le PCR li fana ka lintlha tse khethehileng empa li ja nako, li hloka tsebo, 'me li hloka lisebelisoa tsa laboratori tse tsoetseng pele, tse fokotsang tšebeliso ea tsona e pharaletseng.

● Micro-immunofluorescence (MIF) e fana ka ho nepahala hantle empa e hloka microscope ea fluorescence le tlhaloso ea litsebi, e thibelang tšebeliso ea kamehla.

● ELISA le litlhahlobo tsa khauta tsa colloidal li amoheloa ka ho pharaletseng maemong a kliniki ka lebaka la ho ba bonolo, lebelo, kutloisiso e phahameng le ho khetheha, le boiketlo ba ho bokella sampuli (serum / plasma).

Ts'ebetso ea Sehlahisoa

Likarolo tsa Tlhahlobo ea Beier Bio ea HV-IgM/IgG (ELISA).

● Mofuta oa Mohlala: Serum, plasma

● Sample Dilution: Ka bobeli litlhahlobo tsa IgM le IgG li sebelisa sampole ea mantlha ea seliba e nang le 1:11 dilution (100µl sampole ea diluent + 10µl sampole), e ntlafalletsa tšebetso le ho fokotsa mosebetsi o boima oa opareitara.

● Reagent e Itokiselitse-Ho Sebelisa: Lisebelisoa tsohle li se li lokile ntle le li-buffer tsa ho hlatsoa (20× concentrated). E khethiloe ka mebala bakeng sa ho tsebahatsa habonolo

● Mokhoa oa ho Incubation: 30 min / 30 min / 15 min; e itirisang ka botlalo

● Ho lemoha Wavelength: 450 nm e nang le 630 nm reference

● Lithapo Tse Koaheletsoeng: Liliba tse 96 kapa tse 48 tse robehang, se seng le se seng se na le khoutu ea sehlahisoa se hatisitsoeng bakeng sa ho lateloa le ho ba bonolo.

Likarolo tsa Tlhahlobo ea Beier Bio ea HV-IgM/IgG (Colloidal Gold).

● Mofuta oa Mohlala: Serum

● Nako ea ho Fumana: Liphetho nakong ea metsotso e 15; ha ho hlokahale lisebelisoa tse eketsehileng; e loketse bakeng sa tlhahlobo e potlakileng ho bakuli ba kantle, ba tšohanyetso, le ba hasaneng ba bakuli

● Mokhoa oa ho etsa: Kenya 10µl sampole ho sampole ea karete ea tlhahlobo hantle u sebelisa dropper; hlalosa sephetho nakong ea metsotso e 15–20

Ts'ebetso ea Clinical ea HV-IgM (ELISA), HV-IgG (ELISA), le HV-IgM/IgG (Colloidal Gold)