Herpes Simplex II IgM ELISA Kit

Setsi sena se lemoha Herpes Simplex virus II IgM antibody (HSV2-IgM) ka har'a serum ea motho kapa lisampole tsa plasma, li-polystyrene microwell strips li koahetsoe esale pele ka li-antibodies tse lebisitsoeng ho motho immunoglobulin M protheine (anti-µ chain).Ka mor'a ho qala ho kenyelletsa li-serum kapa tsa plasma tse lokelang ho hlahlojoa, li-antibodies tsa IgM tsa mohlala li ka tšoaroa, 'me likarolo tse ling tse sa tlamisoang (ho kenyeletsa le li-antibodies tse khethehileng tsa IgG) li tla tlosoa ka ho hlatsoa.Mohatong oa bobeli, HRP (horseradish peroxidase) -li-antigen tse kopantsoeng li tla itšoara feela ka li-antibodies tsa HSV2 IgM.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (anti-µ)-(HSV2-lgM)-(HSV2 Ag-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e hokahaneng le e rarahaneng e etsa hore ho be le karabelo ea moqapi oa mebala ho hlahisa ntho e putsoa, ​​eketsa 50 µ I ea Stop Solution, 'me u fetohe mosehla.Boteng ba ho monya ha lesole la 'mele la HSV2-IgM sampoleng bo ile ba khethoa ke sebali sa microplate.