Herpes Simplex kokoana-hloko ea IgM ELISA Kit

Kit ena e lemoha Herpes Simplex Virus I lgM antibody (HSV1-lgM) ka har'a serum ea motho kapa lisampole tsa plasma, li-polystyrene microwell strips li koahetsoe esale pele ka li-antibodies tse lebisitsoeng ho liprotheine tsa motho tsa immunoglobulin M (anti-µ chain).Ka mor'a ho qala ho kenyelletsa li-serum kapa li-plasma tsa ho hlahlojoa, li-antibodies tsa lgM tsa mohlala li ka nkoa, 'me likarolo tse ling tse sa tlangoa (ho kenyeletsa le li-antibodies tse khethehileng tsa lgG) li tla tlosoa ka ho hlatsoa.Mohatong oa bobeli, HRP (horseradish peroxidase) -li-antigen tse kopantsoeng li tla itšoara feela ka li-antibodies tsa HSV1 lgM.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (anti-µ) - (HSV1-lgM) - (HSV1 Ag-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e hokahaneng le e rarahaneng e susumetsa karabelo ea moqapi oa mebala ho hlahisa ntho e putsoa, ​​eketsa 50 µ I ea Stop Solution, 'me u fetohe mosehla.Boteng ba ho monya ha lesole la 'mele la HSV1-lgM sampoleng ho laetsoe ke sebali sa microplate.