Human Cytomegalovirus IgM ELISA Kit

Setsi sena se lemoha sethibela-mafu sa Human Cytomegalovirus IgM (HCMV-IgM) ka har'a serum ea motho kapa lisampole tsa plasma, li-polystyrene microwell strips li koahetsoe esale pele ka li-antibodies tse lebisitsoeng ho motho immunoglobulin M protheine (anti-µ chain).Ka mor'a ho qala ho kenyelletsa li-serum kapa tsa plasma tse lokelang ho hlahlojoa, li-antibodies tsa IgM tsa mohlala li ka tšoaroa, 'me likarolo tse ling tse sa tlamisoang (ho kenyeletsa le li-antibodies tse khethehileng tsa IgG) li tla tlosoa ka ho hlatsoa.Mohatong oa bobeli, HRP (horseradish peroxidase) -antigens conjugated e tla itšoara feela ka li-antibodies tsa HCMV IgM.Ka mor'a ho hlatsoa ho tlosa HRP-conjugate e sa tlamisoang, litharollo tsa chromogen li kenngoa ka har'a liliba.Boteng ba (anti-µ) -(HCMV-lgM) -(HCMV Ag-HRP) immunocomplex, ka mor'a ho hlatsoa poleiti, substrate ea TMB e ile ea eketsoa bakeng sa nts'etsopele ea mebala, 'me HRP e hokahaneng le e rarahaneng e etsa hore ho be le karabelo ea moqapi oa mebala ho hlahisa ntho e putsoa, ​​eketsa 50 µI ea Stop Solution, 'me u fetohe mosehla.Boteng ba ho monya ha antibody ea HCMV-IgM sampoleng ho ile ha khethoa ke sebali sa microplate.