Tlhahlobo ea Tlhahlobo ea TB-IGRA

Tlhaloso e Khutšoanyane:

Teko ea tlhahlobo ea lefuba ea TB-IGRA, eo hape e bitsoang Interferon Gamma Release Assay, ke ELISA bakeng sa tlhahlobo ea bongata ba Interferon Gamma (IFN-γ) e arabelang in vitro stimulation ka Mycobacterium Tuberculosis antigens lisampoleng tsa mali a motho.TB-IGRA e lekanya ts'ebetso ea 'mele ea motho ea ho itšireletsa mafung ho Mycobacterium Tuberculosis.Teko e reretsoe ho sebelisoa e le thuso ho hlahlojoeng tšoaetso ea lefuba ho kenyeletsa le tšoaetso ea lefuba le ipatileng le lefu la Lefuba.

Karabelo ea 'mele ea ho itšireletsa mafung e bakoang ke lefuba la Mycobacterium hangata ke karabelo ea lisele.Ka mor'a tšoaetso ea lefuba la Mycobacterium, 'mele o hlahisa lisele tse khethehileng tsa T tse potolohang maling a peripheral.Tekanyo ea IFN-γ khahlanong le lefuba la Mycobacterium e bontšitsoe e le mokhoa o atlehang oa ho lemoha tšoaetso ea lefuba (ka bobeli e patehileng le e sebetsang), e bitsoang IFN-γ in vitro release assay (IGRA).Phapang e kholo ho tloha tekong ea tuberculin (TST) ke hore IGRA e khetha li-antigen tse khethehileng tse teng feela ho lefuba la Mycobacterium empa li le sieo ho BCG le mycobacteria e ngata e seng lefuba.

Re romelle lengolo-tsoibila

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Molao-motheo

Thepa e nka interferon-γ tokollo assay bakeng sa Mycobacterium tuberculosis (TB-IGRA) ho lekanya matla a karabelo ea 'mele ea ho itšireletsa mafung e kentsoeng ke Mycobacterium tuberculosis specific antigen.
Tekolo ea immunosorbent e amanang le Enzyme le molao-motheo oa sandwich oa antibody.
• Li-microplates li koahetsoe pele ka li-antibodies tsa IFN-γ.
• Mehlala e lokelang ho lekoa e kenngoa ka har'a liliba tsa microplate tse koahetsoeng ke antibody, ebe li-antibodies tsa horseradish peroxidase (HRP) -conjugated anti IFN-γ li kenngoa lilibeng tse fapaneng.
• IFN-γ, haeba e le teng, e tla theha sandwich complex e nang le li-antibodies tsa IFN-γ le li-antibodies tsa HRP-conjugated anti IFN-γ.
• Mmala o tla ntlafatsoa ka mor'a ho kenya litharollo tsa substrate, 'me o tla fetoha ka mor'a ho eketsa litharollo tsa ho emisa.Monyetla (OD) o lekanngoa ka 'mali oa ELISA.
• Khatiso ea IFN-γ ka sampuli e amana le OD e ikemiselitseng.

Likarolo tsa Sehlahisoa

Tlhahlobo e sebetsang ea ELISA bakeng sa ts'oaetso ea lefuba e ipatileng le e sebetsang

Ha ho tšitiso ea ente ea BCG

Tlhaloso ea Sehlahisoa

Molao-motheo	Tekolo ea enzyme e amanang le immunosorbent
Mofuta	Mokhoa oa sandwich
Setifikeiti	CE, NMPA
Mohlala	Madi kaofela
Tlhaloso	48T (bona mehlala ea 11);96T (bona mehlala e 27)
Mocheso oa polokelo	2-8℃
Shelf bophelo	Likhoeli tse 12